Effects of propofol on oxidative stress injury and silent information regulator 1/forkhead box protein 1 pathway of kupffer cells
By: Wen, Jiali
.
Contributor(s): Gu, Shuhan.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2023Edition: Vol.85(4), Jul-Aug.Description: 1110-1117p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: To investigate the effect of propofol on oxidative stress injury and silent information regulator 1/forkhead
box protein 1 pathway of Kupffer cells induced by hydrogen peroxide. Kupffer cells were cultured in vitro,
cell injury model was established by hydrogen peroxide induction, and it was divided into three groups;
hydrogen peroxide injury group (300 μmol·l-1), propofol low (12.5 μg.ml-1), propofol medium (25.0 μg.ml-1),
and propofol high (50.0 μg·ml-1) groups, in addition, Kupffer cells without any treatment were normal control
group. Compared with normal control group ((100.00±0.00) %, (98.19±2.48), (3.76±0.21) pg/ml, (2.46±0.25)
ng/l, (2.47±0.16) pmol/mg, (1.01±0.09), (8.31±0.23), (1.03±0.08), (1.12±0.11), (2.18±0.13)), the Kupffer cell
survival rate (56.24±2.12) %, tetramethylrhodamine ethyl ester fluorescence intensity (54.57±2.64), contents
of superoxide dismutase (2.51±0.19) pg/ml, catalase (1.23±0.14) ng/l, glutathione peroxidase in cell supernatant
(1.31±0.11) pmol/mg, silent information regulator 1 (0.21±0.02), forkhead box protein 1 messenger ribonucleic
acid (0.99±0.03) and protein expressions [(0.19±0.02), (0.11±0.01)) decreased significantly in hydrogen
peroxide group (p<0.05), and the content of malondialdehyde (4.56±0.37) mol/l in supernatant increased
significantly (p<0.05); compared with hydrogen peroxide group, the Kupffer cell survival rate ((64.79±2.49)
%, (72.62±2.53) %, (84.91±2.87) %), tetramethylrhodamine ethyl ester fluorescence intensity [(67.28±2.51),
(80.65±2.64), (94.19±3.05)), contents of superoxide dismutase ((2.89±0.23) pg/ml, (3.24±0.15) pg/ml,
(3.57±0.18) pg/ml), catalase ((1.57±0.18) ng/l, (1.88±0.19) ng/l, (2.23±0.17) ng/l), glutathione peroxidase in cell
supernatant ((1.76±0.15) pmol/mg, (2.03±0.16) pmol/mg, (2.39±0.17) pmol/mg), silent information regulator
1 ((0.39±0.03), (0.52±0.04), (0.86±0.05)), forkhead box protein 1 messenger ribonucleic acid ((2.85±0.27),
(4.11±0.22), (6.19±0.36)) and protein expressions (silent information regulator 1: (0.48±0.04), (0.71±0.06),
(1.01±0.07)), (forkhead box protein 1: (0.45±0.06), (0.64±0.07), (0.87±0.09)) increased significantly in
turn of propofol low group, propofol medium group and propofol high group (p<0.05), and the content
of malondialdehyde ((4.18±0.35) mol/l, (3.62±0.14) mol/l, (2.87±0.17) mol/l) in supernatant decreased
significantly in turn (p<0.05). Propofol can alleviate the oxidative stress injury of Kupffer cells induced by
hydrogen peroxide and promote their survival, which may be related to the regulation of silent information
regulator 1/forkhead box protein 1 pathway.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2024-0421 |
To investigate the effect of propofol on oxidative stress injury and silent information regulator 1/forkhead
box protein 1 pathway of Kupffer cells induced by hydrogen peroxide. Kupffer cells were cultured in vitro,
cell injury model was established by hydrogen peroxide induction, and it was divided into three groups;
hydrogen peroxide injury group (300 μmol·l-1), propofol low (12.5 μg.ml-1), propofol medium (25.0 μg.ml-1),
and propofol high (50.0 μg·ml-1) groups, in addition, Kupffer cells without any treatment were normal control
group. Compared with normal control group ((100.00±0.00) %, (98.19±2.48), (3.76±0.21) pg/ml, (2.46±0.25)
ng/l, (2.47±0.16) pmol/mg, (1.01±0.09), (8.31±0.23), (1.03±0.08), (1.12±0.11), (2.18±0.13)), the Kupffer cell
survival rate (56.24±2.12) %, tetramethylrhodamine ethyl ester fluorescence intensity (54.57±2.64), contents
of superoxide dismutase (2.51±0.19) pg/ml, catalase (1.23±0.14) ng/l, glutathione peroxidase in cell supernatant
(1.31±0.11) pmol/mg, silent information regulator 1 (0.21±0.02), forkhead box protein 1 messenger ribonucleic
acid (0.99±0.03) and protein expressions [(0.19±0.02), (0.11±0.01)) decreased significantly in hydrogen
peroxide group (p<0.05), and the content of malondialdehyde (4.56±0.37) mol/l in supernatant increased
significantly (p<0.05); compared with hydrogen peroxide group, the Kupffer cell survival rate ((64.79±2.49)
%, (72.62±2.53) %, (84.91±2.87) %), tetramethylrhodamine ethyl ester fluorescence intensity [(67.28±2.51),
(80.65±2.64), (94.19±3.05)), contents of superoxide dismutase ((2.89±0.23) pg/ml, (3.24±0.15) pg/ml,
(3.57±0.18) pg/ml), catalase ((1.57±0.18) ng/l, (1.88±0.19) ng/l, (2.23±0.17) ng/l), glutathione peroxidase in cell
supernatant ((1.76±0.15) pmol/mg, (2.03±0.16) pmol/mg, (2.39±0.17) pmol/mg), silent information regulator
1 ((0.39±0.03), (0.52±0.04), (0.86±0.05)), forkhead box protein 1 messenger ribonucleic acid ((2.85±0.27),
(4.11±0.22), (6.19±0.36)) and protein expressions (silent information regulator 1: (0.48±0.04), (0.71±0.06),
(1.01±0.07)), (forkhead box protein 1: (0.45±0.06), (0.64±0.07), (0.87±0.09)) increased significantly in
turn of propofol low group, propofol medium group and propofol high group (p<0.05), and the content
of malondialdehyde ((4.18±0.35) mol/l, (3.62±0.14) mol/l, (2.87±0.17) mol/l) in supernatant decreased
significantly in turn (p<0.05). Propofol can alleviate the oxidative stress injury of Kupffer cells induced by
hydrogen peroxide and promote their survival, which may be related to the regulation of silent information
regulator 1/forkhead box protein 1 pathway.
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